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| Vargas,A.M.; Barros,R.P.A.; Zampieri,R.A.; Okamoto,M.M.; de Carvalho Papa,P.; Machado,U.F.. |
| The GLUT4 transporter plays a key role in insulin-induced glucose uptake, which is impaired in insulin resistance. The objective of the present study was to investigate the tissue content and the subcellular distribution of GLUT4 protein in 4- to 12-year-old control, obese and insulin-treated diabetic mongrel female dogs (4 animals per group). The parametrial white adipose tissue was sampled and processed to obtain both plasma membrane and microsome subcellular fractions for GLUT4 analysis by Western blotting. There was no significant difference in glycemia and insulinemia between control and obese animals. Diabetic dogs showed hyperglycemia (369.9 ± 89.9 mg/dl). Compared to control, the plasma membrane GLUT4, reported per g tissue, was reduced by 55% (P... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Diabetes; Obesity; Insulin resistance; GLUT4; Adipose tissue; Plasma membrane. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700020 |
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| Sabino-Silva,R.; Mori,R.C.; David-Silva,A.; Okamoto,M.M.; Freitas,H.S.; Machado,U.F.. |
| Glucose enters eukaryotic cells via two types of membrane-associated carrier proteins, the Na+/glucose cotransporters (SGLT) and the facilitative glucose transporters (GLUT). The SGLT family consists of six members. Among them, the SGLT1 and SGLT2 proteins, encoded by the solute carrier genes SLC5A1 and SLC5A2, respectively, are believed to be the most important ones and have been extensively explored in studies focusing on glucose fluxes under both physiological and pathological conditions. This review considers the regulation of the expression of the SGLT promoted by protein kinases and transcription factors, as well as the alterations determined by diets of different compositions and by pathologies such as diabetes. It also considers congenital defects... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: SGLT1; SGLT2; SLC5A1; SLC5A2; HNF-1; Diabetes. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001100002 |
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